You don’t need to. It’s just an other file system that you can use instead of the outdated WebDAV (that has also limitations). Just to check if your problem is caused by the ritual file system. I personally preferred WinSFP instead of dokany or WebDAV.
Cryptomator need write read permission to read/write encrypted files. I have to admit that it’s unlikely that you changed by mistake the permission in your vault folder. But it’s not impossible and could be a reason. Just click right on your vault folder (with the Masterkey/vault files in it) and make sure that the permission for your windows user is correct.
And of course you can have a look in the log file and check if there are any suspicious messages when you open the vault and try to read content.